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one-color microarray hybridization protocol, v6.5  (Agilent technologies)


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    Agilent technologies one-color microarray hybridization protocol, v6.5
    One Color Microarray Hybridization Protocol, V6.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+protocol/pm24704562-89-23-24
    Average 90 stars, based on 1 article reviews
    one-color microarray hybridization protocol, v6.5 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    Microarray:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    Article Title: Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties.
    Article Snippet: The quality of the Cy-3 labelled cRNA was checked using NanoDrop, and the yield and specific activity were calculated. .. Hybridization and scanning were done following the protocol described by Agilent using the Agilent G4900DA SureScan Microarray Scanner System. ..

    Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis.
    Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K v2 whole human genome microarray slides (Agilent) at 65 ◦C for 17 h, with a 10 rpm rotation in an Agilent hybridization oven. .. Slides were washed and scanned by the microarray scanner system (Agilent), extracting data through the Feature Extraction 9.5 software (Agilent).

    Article Title: ADRM1-amplified metastasis gene in gastric cancer.
    Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231

    Article Title: Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays
    Article Snippet: Hybridization was performed for 16 hrs at 60°C and arrays were scanned on an Agilent DNA microarray scanner. .. Following the manufacturer's protocol, Agilent's Stabilization and Drying Solution (#5185–5979) was used to protect against the ozone-induced degradation of cyanine dyes on microarray slides during hybridization and processing steps. .. Images were analyzed and data were extracted, background subtracted and normalized using the standard procedures of Agilent Feature Extraction Software A.7.5.1.

    Hybridization:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    Article Title: Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties.
    Article Snippet: The quality of the Cy-3 labelled cRNA was checked using NanoDrop, and the yield and specific activity were calculated. .. Hybridization and scanning were done following the protocol described by Agilent using the Agilent G4900DA SureScan Microarray Scanner System. ..

    Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis.
    Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K v2 whole human genome microarray slides (Agilent) at 65 ◦C for 17 h, with a 10 rpm rotation in an Agilent hybridization oven. .. Slides were washed and scanned by the microarray scanner system (Agilent), extracting data through the Feature Extraction 9.5 software (Agilent).

    Article Title: ADRM1-amplified metastasis gene in gastric cancer.
    Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231

    Article Title: Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays
    Article Snippet: Hybridization was performed for 16 hrs at 60°C and arrays were scanned on an Agilent DNA microarray scanner. .. Following the manufacturer's protocol, Agilent's Stabilization and Drying Solution (#5185–5979) was used to protect against the ozone-induced degradation of cyanine dyes on microarray slides during hybridization and processing steps. .. Images were analyzed and data were extracted, background subtracted and normalized using the standard procedures of Agilent Feature Extraction Software A.7.5.1.

    Labeling:

    Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis.
    Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K v2 whole human genome microarray slides (Agilent) at 65 ◦C for 17 h, with a 10 rpm rotation in an Agilent hybridization oven. .. Slides were washed and scanned by the microarray scanner system (Agilent), extracting data through the Feature Extraction 9.5 software (Agilent).

    Article Title: ADRM1-amplified metastasis gene in gastric cancer.
    Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231

    other:

    Article Title: Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis
    Article Snippet: The glasses were incubated in the Agilent Hybridization Oven at 55 °C, 10 RPM, for 20 h, washed according to the protocol, and scanned using the Agilent DNA Microarray Scanner (G2539C).

    Article Title: Increased Levels of BAFF and APRIL Related to Human Active Pulmonary Tuberculosis
    Article Snippet: The hybridization protocol for the human whole-genome oligonucleotide microarray (Agilent Technologies) and the bioinformatics analysis are shown in (Microarray Test and Bioinformatics Analysis).



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software