one-color microarray hybridization protocol, v6.5 (Agilent technologies)
Structured Review
One Color Microarray Hybridization Protocol, V6.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridization+protocol/pm24704562-89-23-24
Average 90 stars, based on 1 article reviews
Images
Related Articles
Gene Expression:Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Microarray:Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Article Title: Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties. Article Snippet: The quality of the Cy-3 labelled cRNA was checked using NanoDrop, and the yield and specific activity were calculated. .. Hybridization and scanning were done following the protocol described by Agilent using the Agilent Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis. Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K Article Title: ADRM1-amplified metastasis gene in gastric cancer. Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231 Article Title: Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays Article Snippet: Hybridization was performed for 16 hrs at 60°C and arrays were scanned on an Agilent DNA microarray scanner. .. Following the manufacturer's protocol, Agilent's Stabilization and Hybridization:Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Article Title: Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties. Article Snippet: The quality of the Cy-3 labelled cRNA was checked using NanoDrop, and the yield and specific activity were calculated. .. Hybridization and scanning were done following the protocol described by Agilent using the Agilent Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis. Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K Article Title: ADRM1-amplified metastasis gene in gastric cancer. Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231 Article Title: Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays Article Snippet: Hybridization was performed for 16 hrs at 60°C and arrays were scanned on an Agilent DNA microarray scanner. .. Following the manufacturer's protocol, Agilent's Stabilization and Labeling:Article Title: BDE-47, -99, -209 and Their Ternary Mixture Disrupt Glucose and Lipid Metabolism of Hepg2 Cells at Dietary Relevant Concentrations: Mechanistic Insight through Integrated Transcriptomics and Proteomics Analysis. Article Snippet: Briefly, from 1 μg of each RNA sample, we performed cDNA synthesis, cRNA amplification, and labeling by the Quick Amp Labeling Kit Two Colors (Agilent, Santa Clara, CA, USA). .. Samples were alternatively labeled with Cy5 and Cy3 and co-hybridized with a dye swap protocol on 4 × 44 K Article Title: ADRM1-amplified metastasis gene in gastric cancer. Article Snippet: Marlena S. Fejzo,* Lee Anderson, Hsiao-Wang Chen, Adrian Anghel, Jiaying Zhuo, Ravi Anchoori, Richard Roden, and Dennis J. Slamon Division of Hematology-Oncology,Departmentof Medicine,Jonsson Comprehensive Cancer Center,Universityof California at Los Angeles,Los Angeles,CA 90095 Departmentof Oncology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Pathology, the Johns Hopkins University,Baltimore,MD 21231 Departmentof Gynecologyand Obstetrics, the Johns Hopkins University,Baltimore,MD 21231 other:Article Title: Exploring miRNAs’ Based Modeling Approach for Predicting PIRA in Multiple Sclerosis: A Comprehensive Analysis Article Snippet: The glasses were incubated in the Article Title: Increased Levels of BAFF and APRIL Related to Human Active Pulmonary Tuberculosis Article Snippet: The hybridization protocol for the human |
